Multistep downregulation of the secretory pathway in mitosis A fresh Biology Diagrams Misregulation of mitotic entry can often lead to oncogenesis or cell death. Recent research has focused on discovering the signaling pathways that feed into the core checkpoint control mechanisms dependent on Cdk and PP2A. Herein, we review the conserved mechanisms of the G2/M transition, including recently discovered upstream signaling
In search for a mechanistic explanation for the decrease in kindlin protein levels at mitotic entry, we identified several steps of a signalling pathway (Fig. 7f), in which the M-phase-specific
lncreased risk of slippage upon disengagement of the mitotic checkpoint ... Biology Diagrams
Mitotic entry is initiated at late G2 by the cyclin B/CDK1 complex (Takizawa and Morgan, Differences in peak target gene expression may be due to crosstalk with other signalling pathways as well as to a negative feedback loop promoted by Axin2 (Davidson et al, 2009; Hadjihannas et al, 2012). Abrogated or compromised G2 checkpoint will allow premature mitotic entry of defective cells that fail to undergo proper chromosome segregation thereby leading to mitotic catastrophe (Figure 2). In support of this, the fusion of interphase and mitotic cells led to mitotic catastrophe which was due to the cyclin B/cdk1 driven-premature entry of Below we examine these G2 and mitotic signaling pathways and the checkpoints that regulate them, explaining how they ensure that events take place in the correct order even in the face of cell-cycle disruptions. Moseley JB, Mayeux A, Paoletti A, Nurse P 2009. A spatial gradient coordinates cell size and mitotic entry in fission yeast
Later work suggested the presence of pathways that trigger adaptation during protracted arrest Localization of Mad2 was used as a read-out for checkpoint signaling, indefinitely. Cells carry Mad2-GFP to record checkpoint activation status, and Clb2-mCherry to record both mitotic entry (Clb2 rise time) and mitotic exit (Clb2 degradation
Cell cycle checkpoint regulators reach a zillion Biology Diagrams
Therefore, depending on the history of a G2 cell, different pathways within the mitotic entry network will be of different importance for the decision to enter mitosis. We therefore propose that there is little upstream or downstream in the signal transduction within the mitotic entry network, but rather a large spiral of events that slowly
